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Effect of PINCH1 on the function of <t>HL1</t> cells. (A) Successful PINCH1 shRNA was confirmed by western blot analysis. (B) Percentage of GFP + cells in PINCH1 shRNA/PINCH1 OE cells following transfection. Untreated cells were used as a negative control. (C) The expression levels of PINCH1 mRNA in control, PINCH1 shRNA, and PINCH1 shRNA/PINCH1 OE cells were determined by qPCR. (D) HL1 cell viability (magnification, x100) and (E) migration ability were decreased following PINCH1 shRNA (magnification, x100). (F) HL1 cell apoptosis rate was significantly increased following PINCH1 shRNA. ** P<0.05 vs. shRNA NC; ## P<0.05 vs. PINCH1 SHRNA/PINCH1 OE group. PINCH1, particularly interesting new cysteine histidine rich 1; PINCH1 shRNA, PINCH1 knockdown; PINCH1 OE, PINCH1 overexpression; PINCH1 SHRNA/PINCH1 OE, PINCH1 overexpression (PINCH1 OE) in PINCH1 shRNA HL1 cells.
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Effect of PINCH1 on the function of HL1 cells. (A) Successful PINCH1 shRNA was confirmed by western blot analysis. (B) Percentage of GFP + cells in PINCH1 shRNA/PINCH1 OE cells following transfection. Untreated cells were used as a negative control. (C) The expression levels of PINCH1 mRNA in control, PINCH1 shRNA, and PINCH1 shRNA/PINCH1 OE cells were determined by qPCR. (D) HL1 cell viability (magnification, x100) and (E) migration ability were decreased following PINCH1 shRNA (magnification, x100). (F) HL1 cell apoptosis rate was significantly increased following PINCH1 shRNA. ** P<0.05 vs. shRNA NC; ## P<0.05 vs. PINCH1 SHRNA/PINCH1 OE group. PINCH1, particularly interesting new cysteine histidine rich 1; PINCH1 shRNA, PINCH1 knockdown; PINCH1 OE, PINCH1 overexpression; PINCH1 SHRNA/PINCH1 OE, PINCH1 overexpression (PINCH1 OE) in PINCH1 shRNA HL1 cells.

Journal: Experimental and Therapeutic Medicine

Article Title: PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF-κB signaling pathway

doi: 10.3892/etm.2021.10984

Figure Lengend Snippet: Effect of PINCH1 on the function of HL1 cells. (A) Successful PINCH1 shRNA was confirmed by western blot analysis. (B) Percentage of GFP + cells in PINCH1 shRNA/PINCH1 OE cells following transfection. Untreated cells were used as a negative control. (C) The expression levels of PINCH1 mRNA in control, PINCH1 shRNA, and PINCH1 shRNA/PINCH1 OE cells were determined by qPCR. (D) HL1 cell viability (magnification, x100) and (E) migration ability were decreased following PINCH1 shRNA (magnification, x100). (F) HL1 cell apoptosis rate was significantly increased following PINCH1 shRNA. ** P<0.05 vs. shRNA NC; ## P<0.05 vs. PINCH1 SHRNA/PINCH1 OE group. PINCH1, particularly interesting new cysteine histidine rich 1; PINCH1 shRNA, PINCH1 knockdown; PINCH1 OE, PINCH1 overexpression; PINCH1 SHRNA/PINCH1 OE, PINCH1 overexpression (PINCH1 OE) in PINCH1 shRNA HL1 cells.

Article Snippet: To determine HL1 cell viability, a Cell Counting Kit 8 (CCK-8) assay was used according to the manufacturer's instructions (Beyotime Institute of Biotechnology).

Techniques: shRNA, Western Blot, Transfection, Negative Control, Expressing, Control, Migration, Knockdown, Over Expression

HL1 cell apoptosis is associated with the NF-κB signaling pathway. Expression levels of NF-κB signaling pathway-related proteins were (A) determined by western blotting and (B) semi-quantified. NF-κB, MYD88, TNF-α and caspase-3 expression levels were significantly increased, whereas MMP-2 and MMP-9 expression levels were notably decreased in the shRNA PINCH1 group. ** P<0.01 vs. shRNA NC group. MYD88, myeloid differentiation factor 88; PINCH1, particularly interesting new cysteine histidine rich 1; WT, wild-type; shRNA, short hairpin RNA.

Journal: Experimental and Therapeutic Medicine

Article Title: PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF-κB signaling pathway

doi: 10.3892/etm.2021.10984

Figure Lengend Snippet: HL1 cell apoptosis is associated with the NF-κB signaling pathway. Expression levels of NF-κB signaling pathway-related proteins were (A) determined by western blotting and (B) semi-quantified. NF-κB, MYD88, TNF-α and caspase-3 expression levels were significantly increased, whereas MMP-2 and MMP-9 expression levels were notably decreased in the shRNA PINCH1 group. ** P<0.01 vs. shRNA NC group. MYD88, myeloid differentiation factor 88; PINCH1, particularly interesting new cysteine histidine rich 1; WT, wild-type; shRNA, short hairpin RNA.

Article Snippet: To determine HL1 cell viability, a Cell Counting Kit 8 (CCK-8) assay was used according to the manufacturer's instructions (Beyotime Institute of Biotechnology).

Techniques: Expressing, Western Blot, shRNA